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. 2026 Jan 13;36:e2601001. doi: 10.4014/jmb.2601.01001

Fig. 1. Proteomic analysis of skin ECM reveals skin-mimetic ECM components and functional properties required for the skin microenvironments.

Fig. 1

(A) PCA based on the total protein profiles of human skin tissue, skin ECM, rhCollagen, and VitroCol. (B) Pie charts depicting the relative proportions of matrisome subcategories in human skin tissue, skin ECM, rhCollagen, and VitroCol. (C) Relative abundance of collagen subtypes in human skin tissue, skin ECM, rhCollagen, and VitroCol. Top 10 most abundant (D) glycoproteins and (E) proteoglycans in skin ECM. (F) Venn diagrams showing the number of shared and unique proteins between human skin tissue and skin ECM. (G) Proportion of skin-expressed proteins among matrisome proteins identified in skin ECM (approximately 99.12% of the total matrisome protein content). Skin-expressed proteins were defined by the Human Protein Atlas. Top 10 most significantly enriched gene ontology terms for (H) biological process and (I) molecular function based on the skin-expressed matrisome protein set shown in (G). (J) Heatmap showing the expression of proteins associated with extracellular matrix organization (GO:0030198) in skin ECM (n = 3). Proteins are categorized into collagens, glycoproteins, proteoglycans, matrisome-associated proteins (ECM regulators, ECM-affiliated proteins, and secreted factors), and non-matrisome proteins. The heatmap is divided to reflect these classifications, with proteins within each category arranged in descending order of abundance. PPI networks of proteins associated with (K) elastic fiber assembly (GO:0048251) and (L) cell redox homeostasis (GO:0045454) in skin ECM. Node color indicates relative protein abundance.