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. 2026 Jan 13;36:e2601001. doi: 10.4014/jmb.2601.01001

Fig. 4. Suppression of α-MSH-induced melanogenic transcripts and melanin accumulation by skin ECM.

Fig. 4

(A) qPCR analyses of Tyr1, Tyrp1, and Tyrp2 mRNA levels in B16F10 cells under untreated control, α-MSH stimulation (100 nM), and skin ECM pretreatment (25 μg/ml) followed by α-MSH stimulation, measured at 72 h (n = 3, ns: not significant, *p < 0.05, **p < 0.01, and ****p < 0.0001 versus indicated condition). (B) Relative intracellular melanin content in B16F10 cells following α-MSH stimulation (100 nM) after pretreatment with skin ECM (25 μg/ml), RP (10 μg/ml), PDRN (25 μg/ml), rhCollagen (25 μg/ml), and VitroCol (25 μg/ml) (n = 3, *p < 0.05, ***p < 0.001, and ****p < 0.0001 versus indicated condition). (C) Relative intracellular melanin content in B16F10 cells following α-MSH stimulation (100 nM) after pretreatment with skin ECM (25 μg/ml), HDF exosome (1 × 105 particles/ml), and CAcEV (1 × 105 particles/ml) (n = 3, *p < 0.05 and ***p < 0.001 versus indicated condition). Data are presented as mean ± SD. Statistical significance was analyzed using one-way ANOVA with Tukey’s multiple comparisons test.