Fig. 2. PVT-1 enhances osteosarcoma growth and metastatic potential.

a Cell viability assessment secondary to PVT-1 overexpression in HOS cells. b Cell viability assessment secondary knockdown of PVT-1 in TCCC-OS63. c Analysis of in vitro migration and invasion characterization for HOS-PVT1 o.e. cell lines by trans-well migration and invasion assay. d Migration and invasion analysis for TCCC-OS63-shPVT-1 cell line. Error bars represent S.D.; a Student’s t test or one-way ANOVA was used to calculate statistical significance values for all figures. *p < 0.05; **p < 0.001; ***p < 0.0001. Data represent three independent experiments.