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. Author manuscript; available in PMC: 2026 Jan 24.
Published in final edited form as: Oncogene. 2022 Nov 8;41(50):5373–5384. doi: 10.1038/s41388-022-02538-w

Fig. 3. PVT-1 induces cancer stem-like phenotypes.

Fig. 3

a RT-qPCR analysis for expression of stem cell genes in HOS PVT-1 Ctrl and PVT-1 o.e. cells. b Western blot analyzed the stem cell protein level in HOS PVT-1 Ctrl and PVT-1 o.e. cells. c Quantification of serial dilution secondary and tertiary sarcospheres from HOS PVT-1 Ctrl and PVT-1 o.e. cells. HOS PVT-1 Ctrl: 100 and 10 cell group developed no secondary sarcospheres. None detected (N.D.). Secondary sarcospheres were generated from the primary sarcosphere and secondary sarcospheres were used for tertiary sarcosphere production. d Expression level of PVT-1 in HOS adherent and HOS sarcospheres. *p < 0.05; **p < 0.001; ***p < 0.0001. Data represent three independent experiments.