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. Author manuscript; available in PMC: 2026 Jan 24.
Published in final edited form as: Oncogene. 2022 Nov 8;41(50):5373–5384. doi: 10.1038/s41388-022-02538-w

Fig. 4. PVT-1 drives osteosarcoma cancer stem-like behavior through TSC2.

Fig. 4

a RPPA analysis of HOS Ctrl and PVT-1 o.e. cells. b GSEA analysis of the RPPA data identified cellular pathways impacted by the enhancement of PVT-1. c Western blot analysis of TSC2 in HOS Ctrl and PVT-1 o.e. cells. d RT-qPCR of TSC2 in HOS Ctrl and PVT-1 o.e. cells. e–h Rescue experiments to understand the role of TSC2 as a downstream effector of PVT-1. Effects of TSC2 overexpression in HOS PVT-1 o.e. cell lines on: e Cell viability. f Migration and invasion assay. g Stem cell markers via western blot. h Serial-Dilution Sphere formation. None detected (N.D.). Error bars represent S.D. Statistical analysis was performed using linear regression of e and t-test or one-way ANOVA for the rest of the figures. *p < 0.05; **p < 0.001; ***p < 0.0001. Data represent three independent experiments.