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. Author manuscript; available in PMC: 2026 Jan 24.
Published in final edited form as: Oncogene. 2022 Nov 8;41(50):5373–5384. doi: 10.1038/s41388-022-02538-w

Fig. 7. PVT-1/TRIM28 axis is associated with TSC2 ubiquitination.

Fig. 7

HOS cell lines transfected with PVT-1 o.e. or shTRIM28 are labeled with a “+” and if the corresponding control plasmid was inserted with a “−” a Western blot to detect TSC2 level dependent upon the perturbation of PVT-1 and/or TRIM28 in HOS cells. b HOS cells were transiently transfected with HA-Ubiquitin plasmid. 48 h post-transfection, endogenous TSC2 was immunoprecipitated from whole cell lysate and ubiquitinated TSC2 was detected by western blot using anti-HA antibody. c Western blot to determine presence of Vps34 co-immunoprecipitates: SUMO1, TRIM28, and TSC1 in HOS cells. d Cells were treated with MG132 at 10 uM for 5 h. Whole cell lysates of these cells were used for TSC1 co-immunoprecipitation. Immunoblots were analyzed for detection of TSC2 and Vps34. e CCK-8 assessment of HOS PVT-1 o.e. cell sensitivity to SUMOylation inhibitor. f Proposed model of PVT-1/TRIM28 signaling cascade which promotes tumorigenic and cancer-stem like phenotypes. Error bars represent S.D. Statistical analysis was performed using one-way ANOVA. *p < 0.05; **p < 0.001; ***p < 0.0001. Data represent three independent experiment.