TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| C. albicans strains | ||
| CAF2-1 | URA3/ura3Δ::imm434 | 10 |
| CAI4 | ura3Δ::imm434/ura3Δ::imm434 | 10 |
| C4-SB1, C4-SB4 | as CAI4, but SIT1/sit1Δ::hisG-URA3-hisG | This study |
| C4-SH1.1, C4-SH4.1 | as CAI4, but SIT1/sit1Δ::hisG | This study |
| C4-SHB1.1 | as CAI4, but sit1Δ::hisG-URA3-hisG/sit1Δ::hisG | This study |
| C4-SHH1.1 | as CAI4, but sit1Δ::hisG/sit1Δ::hisG | This study |
| C4-SHB4.21 | as CAI4, but SIT1/sit1Δ::hisG-URA3-hisG/sit1Δ::hisG | This study |
| C4-SHH4/21-VII | as CAI4, but SIT1/sit1Δ::hisG/sit1Δ::hisG | This study |
| C4-SHHB4/21-VII | as CAI4, but sit1Δ::hisG-URA3-hisG/sit1Δ::hisG/sit1Δ::hisG | This study |
| C4-SHHH4/21-VII | as CAI4, but sit1Δ::hisG/sit1Δ::hisG/sit1Δ::hisG | This study |
| SM1813C | integration of p1367-CaSITpORF/ClaI in C4-SHH1.1 | This study |
| SM1814C | integration of p1367-CaSITp/ClaI in C4-SHH1.1 | This study |
| ΔCaftr1 (ura+) | as CAI4, but ftr1Δ::hisG ftr1Δ::hisG ftr1Δ::hisG-URA3-hisG | 33 |
| Fet- | as CAI4, but fet3Δ::hisG fet3Δ::hisG-URA3-hisG | 9 |
| S. cerevisiae strains | ||
| DEY1394 | MATα ade6 can1 his3 leu2 trp1 ura3 fet3-2::HIS3 | 2 |
| PHY14 | as DEY1394, but arn1Δ::loxP arn2Δ::loxP sit1Δ::loxP enb1Δ::loxP-kanMX-loxP | This study |
| C. albicans plasmids | ||
| p1367/1 | URA3-marked integrative vector | 29 |
| p1367-CaSITpORF | SIT1 promoter and ORF in p1367 | This study |
| p1367-CaSITp | SIT1 promoter in p1367 | This study |
| S. cerevisiae plasmids | ||
| YCp22Gal | GAL1/10 promoter in YCplac22 | This study |
| YCp22Gal/SIT1 | SIT1 transcribed by GAL1 promoter, in YCp22 | This study |