FIG. 1.

Time course of O. tsutsugamushi-stimulated chemokine induction in HUVEC. (A) The levels of chemokine mRNAs at each time point were assayed by RNase protection. (B) The mRNA levels of MCP-1 gene were analyzed by semiquantitative RT-PCR in 1.2% agarose gel. The levels of β-actin transcripts were also determined to normalize the transcripts of MCP-1. (C) The band densities were determined with TINA software, and the mRNA expression level for MCP-1 was normalized with respect to the intensities of the bands of β-actin. Abbreviations: MIP-1α, macrophage inflammatory protein 1α; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.