FIG. 2.

EMSA showing activation of transcription factors, NF-κB (A) and AP-1 (B), in O. tsutsugamushi-infected HUVEC at each time point. A competitive inhibition assay was performed with nuclear extracts that were preincubated with 50-fold molar excesses of either unlabeled NF-κB (NF) or AP-1 (AP) consensus oligonucleotide.