FIG. 7.

Mutational analysis of transcriptional control elements in the MCP-1 enhancer region. (A) A series of clones was used to characterize the MCP-1 enhancer region. pMCP560-En contains insert DNA as described in the legend to Fig. 6. Nucleotides of the NF-κB consensus site (hatched box) and/or AP-1 consensus site (filled box) in the enhancer region were substituted as described in Material and Methods and are indicated (X). (B) Luciferase activity of HUVEC transfected transiently with clones in panel A. The extracts from cells stimulated with O. tsutsugamushi (closed bars) or left untreated (open bars) were assayed for luciferase activity. Luciferase activity was normalized as described in the legend to Fig. 6. Results are given as means + standard deviations (error bars) of three independent transfection experiments.