FIG. 1.

Schematic representation of the cloning strategy used to construct plasmids for complementing a ΔfurA-katG strain of M. tuberculosis with different katG alleles. Site-directed mutagenesis of katG was carried out with plasmid pKATII. An NdeI-MluI fragment spanning the point mutations was subsequently transferred to the equivalent sites in pPD28 to create pAP21 to pAP23. pPD28 is based on pKINT, a Bluescript-based mycobacterial integration vector. The boldface arrow marked P denotes the site of the main katG promoter in M. tuberculosis.