TABLE 1.

Principal bacterial strains and plasmids used in this study

Bacterium or plasmid	Genotype or relevant features	Reference
Bacteria
INH34	ΔfurA-katG strain of M. tuberculosis	30
H37Rv	Genome sequenced strain of M. tuberculosis	5
Plasmids
pKATII	E. coli expression vector containing katG of M. tuberculosis	33
pK275P	pKATII with T275P mutation in katG	33
pK139V	pKATII with A139V mutation in katG	This study
pK315T	pKATII with S315T mutation in katG	33
pKINT	Mycobacterial integration vector	30
pPD28	pKINT with 4.8-kb fragment spanning the furA-katG locus of M. tuberculosis	30
pAP01	pPD28 with deletional interruption of katG	30
pAP21	pPD28 with T275P mutation in katG	This study
pAP22	pPD28 with A139V mutation in katG	This study
pAP23	pPD28 with S315T mutation in katG	This study