TABLE 3.
Biochemical properties of the TB10.4 family membersa
| Gene | Antigen | No. of aa/ no. of bases | Molecular mass (kDa) | pI | % Homology to TB10.4b | Homologous protein in:
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CSU#93c
|
M. bovisd
|
M. aviume,f
|
|||||||||
| Status TIGR ID | % Homology | Status | % Homology | Status | % Homology | ||||||
| Rv 0288 | TB10.4 | 96/291 | 10.391 | 4.43 | 100 (96/96) | Yes, MT0301 | 100 (96/96) | Yes | 100 (96/96) | Yesf | 79 (76/96) |
| Rv3019c | TB10.3 | 96/291 | 10.313 | 4.2 | 84 (81/96) | Yes, MT3104 | 100 (96/96) | Yes | 100 (96/96) | Yesf | 80 (77/96) |
| Rv3017c | TB12.9 | 120/363 | 12.923 | 7.92 | 65 (45/69) | Yes, MT3097 | 100 (120/120) | Yes | 89 (107/120) | No | 62 (44/70) |
aa, amino acids. Numbers in parentheses refer to the number of homologous amino acids/total number of amino acids.
Determined by database BLASTP searches at http://www.sanger.ac.uk/Projects/M_tuberculosis/blast_server.shtml.
Determined by database BLASTP searches at http://198.76.161.137/cmr-blast/Index.cgi?database=GMT.pep. TIGR ID refers to the TIGR database identification number.
Determined by database BLASTP searches at http://www.sanger.ac.uk/Projects/M_bovis/blast_server.shtml.
Determined by database BLASTP searches at http://www.tigr.org/cgi-bin/BlastSearch/blast.cgi?organism=m_avium.
Only one copy of the TB10.4 family is present in M. avium (Table 2). It is not possible to determine if this protein is the homologue of TB10.4 or TB10.3.