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. Author manuscript; available in PMC: 2005 Nov 14.
Published in final edited form as: J Biol Chem. 2005 Mar 8;280(19):18822–18832. doi: 10.1074/jbc.M501757200

Fig. 7. Recovery of 11-cis-retinal after prolonged and intense bleaching in prRDH+/+ and prRDH−/− mice.

Fig. 7

A, dark-adapted mice were exposed to background light of 500 cd·m−2 for 3 min and returned to the dark. Retinoid analysis by HPLC was performed at 0 and 30 min after the bleach. B, dark-adapted mice were exposed to background light of 500 cd·m−2 for 3 min and returned to the dark. Retinoid analysis by HPLC was performed 30 min after the bleach separately for the retina and the RPE. There is partial unavoidable cross-contamination of the RPE and the retina as measured by the presence of retinyl esters in the retinal fraction and 11-cis-retinal in the RPE. Note that retinal isomers were converted into oximes with hydroxylamine before HPLC separation.