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. Author manuscript; available in PMC: 2005 Nov 14.
Published in final edited form as: J Biol Chem. 2002 Sep 12;277(47):45195–45202. doi: 10.1074/jbc.M205380200

Fig. 3. Northern blot analysis of mouse suprabasin.

Fig. 3

A, expression of suprabasin in the epidermis of newborn mice. Northern blot of poly(A)+ mRNA (2 μg) from basal and differentiated keratinocytes isolated by discontinuous Percoll gradient from neonatal epidermis. The position of the 18 and 28 S ribosomal RNAs are shown. B, expression of suprabasin in primary mouse keratinocytes cultured and differentiated in vitro by addition of Ca2+. C, time course of suprabasin expression in cultured mouse keratinocytes and after 24 h of treatment with different kinase inhibitors: GF (PKC inhibitor), H89 (PKA inhibitor), or KN62 (CaM kinase II inhibitor). D, mouse embryonic mRNA blot (Clontech). Panel shows 2 μg of poly(A)+ per lane from four embryonic developmental stages (7-, 11-, 15-, and 17-day embryos). RNA size marker is indicated on the left. E, adult multiple tissue mRNA Northern blot (Clontech). Tissue sample is indicated above each lane. All blots were hybridized with 262-bp suprabasin, glyceraldehyde-3-phosphate dehydrogenase, and PL-1 (placenta lactogen 1) probes, as indicated.