FIG. 4.
Parasitemia and mortality in genetically immunized mice. C57BL/6 mice were injected with CMVI.UBF3/2 encoding ASP-1, ASP-2, or TSA-1, with (C and D) or without (A and B) cytokine adjuvants, twice at an interval of 6 weeks. Mice injected with the empty CMVI.UBF3/2 plasmid were used as controls. Two weeks after the second immunization, mice were challenged with a lethal dose of T. cruzi (105 BFT/mouse). Blood parasite levels (A and C) were monitored at weekly intervals, and survival (B and D) was recorded daily.