FIG. 6.
Inflammation and tissue parasitemia in immunized and challenged mice. C57BL/6 mice were immunized with empty CMVI.UBF3/2 (A), with cytokine plasmids (B), with ASP-1-, ASP-2-, TSA-1-encoding plasmids (C), or with a mixture of antigen-encoding plasmids plus cytokine-expressing plasmids (D) twice at 6-week intervals. Mice were infected 2 weeks after their second immunization with a lethal dose of T. cruzi BFT (105/mouse). Skeletal muscle sections for histologic analysis were obtained at 45 days postinfection. Parasite-infected cells are indicated by arrows. Magnification, ×20.