TABLE 3.

Lysis of various erythrocytes by ClyA_STy and ClyA_EC

Strain	Plasmid charac- teristics	Lysis (% of maximum)a
		Horse	Sheep	Human	Goat	Hen	Rabbit
DH5α/pUC18	p(vector)	1.6	1.6	1.1	0.7	1.0	0.1
DH5α/pYMZ81	p(ClyAEC)	96	82	69	46	43	10
DH5α/pGEM-T Easy	p(vector)	2.5	0.9	0.6	0.9	2.7	0.1
DH5α/pJON97	p(ClyASTy)	57	37	35	30	21	0.5

^aLysis of erythrocytes was quantified using the hemolysis assay described in Materials and Methods. Shown are the percentage as of the maximal release of hemoglobin which was obtained by the addition of 1% Triton X-100 in the reaction wells. The bacteria were grown on LB agar plates at 37°C for 16 to 17 h before being harvested. Approximately 2 × 10⁷ bacterial cells were used for the samples applied in each assay well (final density, 2 × 10⁸ bacterial cells ml⁻¹).