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. Author manuscript; available in PMC: 2005 Nov 14.
Published in final edited form as: Nat Struct Mol Biol. 2004 Oct 10;11(11):1076–1083. doi: 10.1038/nsmb845

Figure 4.

Figure 4

Quantification of overlap between histone modifications and CENP-A/CID on metaphase chromosomes from humans and flies. Metaphase chromosomes from human cells and flies were analyzed by fluorescence line plots to determine the amount of overlap between CENP-A/CID and modified histone antibody signals. Overlap between CENP-A or CID staining, and H3 Lys9-diMe, was less than that seen for H3 Lys4-diMe. More overlap was observed in metaphase chromosomes in comparison with fibers for Lys9 methylation and some of the acetylated H3 and H4 residues, reflecting the lower resolution obtained in condensed chromosomes versus extended fibers.