Figure 1. Northern blot analysis for steady-state LO mRNA levels in control and CSC-treated cells.
Total RNA (15 μg) was derived from growth-arrested cells treated with or without CSC for 24 h at indicated doses. Transcripts for LO and GAPDH, an internal control, migrated and labeled as shown. Densities of LO bands were normalized to internal controls following analysis by the 1D Scan EX software. Experiments shown here and below were repeated at least three times with reproducible results, and a representative one is presented, unless otherwise indicated.