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. Author manuscript; available in PMC: 2006 Sep 1.
Published in final edited form as: Toxicol Sci. 2005 Jun 2;87(1):197–203. doi: 10.1093/toxsci/kfi212

Figure 2. Nuclear run-on assay for relative transcription rates of LO in control and CSC-treated cells.

Figure 2

Nuclei were freshly isolated from control and CSC-treated cells under the same conditions as described in Figure 1. Nascent transcripts were labeled with 32P-UTP and hybridized to a previously prepared filter containing cDNAs for LO, histone 3B (H3B) and glyceraldehydes-3-phosphate dehydrogenase (GAPDH) and pUC plasmids without insert (pUC).