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. Author manuscript; available in PMC: 2006 Sep 1.
Published in final edited form as: Toxicol Sci. 2005 Jun 2;87(1):197–203. doi: 10.1093/toxsci/kfi212

Figure 4B. LO promoter activities in control and CSC-treated cells.

Figure 4B

RFL6 cells were co-transfected with LO promoter-reporter plasmids and pSV-β-galactosidase (β-gal) vectors. After 6 h posttransfection, cells were washed, incubated in 10% FBS/DMEM for an additional 18 h period, washed again, and exposed to CSC at indicated doses for 24 h. Luciferase and β-gal activities in control and treated cells were measured according to manufacturer’s instructions. LO-promoter activities in transfected cells were normalized to β-gal activities and expressed as cpm/OD β-gal (100% luciferase activity in the control = 3,528 ± 312 cpm/OD β-gal). ** p < 0.01 vs. control for cells treated with 80 and 120 μg/ml of CSC .