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SDS-PAGE analysis of native SLPs extracted from C. difficile strains 1, 17, and 630; recombinant SlpA precursor expressed in L. lactis (pNZ-1e4H); and recombinant high- and low-MW subunits expressed in E. coli (pET28-High and pET28-Low). The last three samples were purified on Ni-NTA agarose. Molecular mass standards in kilodaltons are indicated on the left.
