FIG. 3.

Countour plots illustrating the staining of integrin β₇ versus that of CD45RA. Peripheral blood mononuclear cells were purified by magnetic beads into CD4⁺ (A)- and CD8⁺ (D)-T-cell fractions. The cells were stained with antibodies to β₇ and CD45RA and were sorted by flow cytometry into CD45RA⁻ β₇^high (B and E) and CD45RA⁻ β₇⁻/CD45RA⁺ β₇^intermediate (C and F) populations. The percentages of the populations are shown at the gates; each figure contains data from at least 1,000 cells and depicts results of one representative experiment out of 15 (A to C) or 13 (D to F).