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Immunodetection of ET by the anti-mORF1 antibody. Purified recombinant ETA, ETB, and mORF1 were resolved on an SDS-12% PAGE gel and either stained with Coomassie brilliant blue (a) or transferred to a nitrocellulose membrane (b through d). The membrane was subjected to immunodetection by an anti-ETA antibody (b), anti-ETB antibody (c), or anti-mORF1 antibody (d). Lanes 1, ETA (0.7 μg); lanes 2, ETB (0.7 μg); lanes 3, mORF1 (0.6 μg).
