FIG. 8.
In vitro treatment of baculovirus-expressed recombinant desmogleins by ETD. Approximately 1 μg of the purified extracellular domain of mouse (left) or human (right) Dsg1 or Dsg3 produced by a baculovirus was incubated with various concentrations of ETD. The extracellular domain of Dsg1, but not that of Dsg3, was cleaved by ETD in a dose-dependent fashion. Arrows point to the 29-kDa degraded band of Dsg1. Bars on the left side indicate molecular size standards of 150, 100, 50, and 25 kDa (from top to bottom).
