FIG. 9.

PFGE and genotypic and phenotypic characterizations of edin-B-positive S. aureus strains. SmaI-digested DNA of edin-B-positive strains was subjected to PFGE and was run on agarose gels. Gels were subjected to Southern hybridization with probes for detecting genes of interest. DNA fragments that hybridized with the probe for detecting the gene of interest are boxed in green (etd and edin-B), yellow (seb), or red (sec). The hybridization signals for edin-B and etd corresponded to the same ca. 140-kb DNA fragment, and that for an enterotoxin gene (seb or sec) corresponded to a 320- to 350-kb DNA fragment. At the bottom, results of coagulase serotyping and data on production of ETD, EDIN-B, enterotoxin, and TSST-1 are summarized. Production of ETD and EDIN-B was assessed by immunoblotting as described in Materials and Methods. Production and serotyping of enterotoxins and TSST-1 were assayed by using the SET-RPLA and TST-RPLA detection kits, respectively. NT, nontypeable. Lanes: 1, TY114; 2, TY129; 3, TY67; 4, TY130; 5, TY134; 6, TY136; 7, TY137; 8, TY140; 9, TY132; 10, TY133; 11, TY135; 12, TY138; 13, TY139; 14, TY141; 15, TY142; 16, TY143; 17, TY144; 18, TY131. Lanes 1 to 3 show results for Japanese strains; lanes 4 to 18 show results for German strains.