TABLE 1.
Primers used in this study
| Gene | Primer | Oligonucleotide sequence (5′→3′) | Location within gene (bp) | Size of amplified product (bp) |
|---|---|---|---|---|
| eta | ET-1 | CTATTTACTGTAGGAGCTAG | 46-65 | 741 |
| ET-2 | ATTTATTTGATGCTCTCTAT | 767-786 | ||
| etb | ET-3 | ATACACACATTACGGATAAT | 167-186 | 629 |
| ET-4 | CAAAGTGTCTCCAAAAGTAT | 776-795 | ||
| ET mixed primer | ET-7 | GCWACHGGWGTDTTAATWGGWAAA | ||
| ET-8 | ATTAAATATACCTGATCCAGA | |||
| etd | ET-14 | AACTATCATGTATCAAGG | 289-306 (5697-5714a) | 376 |
| ET-15 | CAGAATTTCCCGACTCAG | 647-664 (6055-6072a) | ||
| pCL8-etd | ET-18 | ACTCGCATCCATGTTTCC | 5067-5084a | 1,398 |
| ET-19 | GATAGTTAGCAACTAATAG | 6446-6464a | ||
| His6-eta | ET-21 | CCATGGAAGTTTCAGCAGAAGAAATAA | 115-136 | 739 |
| ET-22 | GGATCCTCATTTTTCTCGTTTATAATGCG | 816-840 | ||
| His6-etb | ET-23 | GCATGCAAGAATACAGCGCAGAAGAAATC | 95-117 | 752 |
| ET-27 | GGATCCTTTATCTAATTTTGCTCTCTTTTTCA | 806-831 | ||
| His6-etd | ET-25 | GCATGCAAACATATGAAGAATCTGAAATT | 100-120 (5508-5528a) | 758 |
| ET-26 | AGATCTATGAGACTGTAATTCAGCTCT | 823-843 (6231-6251a) | ||
| edin-B | ednB-1 | CATAAATACTCCTCTAAG | 243-260 (7384-7401a) | 444 |
| ednB-2 | GCATATTCTGTCCCTCTA | 669-686 (7810-7827a) | ||
| tst-1 | TSST-1 | CTGCTACAGATTTTACCC | 59-78 | 477 |
| TSST-2 | GAGTTAGCTGATGACGAA | 518-535 | ||
| sea | sea-1 | CATTGCCCTAACGTTGAC | 33-50 | 665 |
| sea-2 | ATACTGTCCTTGAGCACC | 670-697 | ||
| seb | seb-1 | GTATGGTGGTGTAACTGAGC | 666-685 | 164 |
| seb-2 | CCAAATAGTGACGAGTTAGG | 810-829 | ||
| sec | sec-1 | AGATGAAGTAGTTGATGTGTATGG | 432-455 | 451 |
| sec-2 | CACACTTTTAGAATCAACCG | 863-882 | ||
| sed | sed-1 | CTAGTTTGGTAATATCTCC | 40-58 | 713 |
| sed-2 | ATATGAAGGTGCTCTGTGG | 734-752 | ||
| Others | ET-28 | TATGGTCGATTGCGAATG | 5093-5110a | |
| ET-29 | CTACACAAGTACTTACACCG | 7979-7998a | ||
| ET-40 | CTCATATAGGTTATATTTTG | 8775-8794a | ||
| ET-85 | CATGTACATCAGACTGCCTTGTATAAGATTTGAAA | 665-699a | 9,432 | |
| ET-86 | GATGTCGAATAGTAGAGATGAAGCATTAAAGCAGA | 10062-10096a |
a
Location within 14,849-kb etd pathogenicity island.