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Primers used for semiquantitative RT-PCR
| Gene | Primers (5′ → 3′) (forward above reverse) | Product size (bp) |
|---|---|---|
| IL-8 | TGCCTCATGTACTGTGTGGG | 286 |
| GGGATAAAGAAACCAAGGCG | ||
| IL-10 | GTTGCCTGGTCTTCCTGGCTG | 482 |
| TATGTAGTTGATGAAGATGTC | ||
| IL-12 | GAGGATTTTCCTCCCAAAGC | 125 |
| CTTCTACAATGGGGCGTGTT | ||
| IFN-γ | TGTGATGTGCTCCATCCTGT | 164 |
| ATTCTGCTGTCTAGGCCCAA | ||
| TNF-α | TGGAGGGAGAAGGGATTCTT | 282 |
| AGGGGCTGGAGAAAGGGAAG | ||
| GM-CSF | CGTCTCTGAAAAGTTTGACTCCC | 300 |
| ACTTCTGGGCTGGTTCCC | ||
| β-Actin | ATCAAGCCCACAGGTACTGG | 141 |
| TCACATAGTGAGGCCGAGTG |
