FIG. 1.
Vaccination status affects IL-8 mRNA production by alveolar macrophages. In vitro-cultured alveolar macrophages from nonvaccinated (open bars) and vaccinated (hatched bars) guinea pigs were infected with live M. tuberculosis H37Ra (B) or H37Rv (MOI, 1:1) (C) or cultured with or without LPS (10 μg/ml) (A) for 3, 12, and 24 h. Total RNA from the macrophages was collected, reverse transcribed to cDNA, and detected by real-time PCR by using probes for IL-8 and 18s rRNA. Fold induction was determined from Ct values normalized for 18s expression and then normalized to the value derived from nonvaccinated controls. Alveolar macrophages isolated from vaccinated animals produced significantly more RNA for IL-8 when stimulated with LPS (A) or infected with M. tuberculosis H37Ra (B) and H37Rv (C) at 12 and 24 h poststimulation than did alveolar macrophages isolated from nonvaccinated guinea pigs. Data are means ± standard errors of the means of four animals. P values of <0.05 (*) were considered significant.