FIG. 3.

Effects of vaccination and in vitro stimulus on IL-8 production. Alveolar macrophages isolated from nonvaccinated and BCG-vaccinated guinea pigs were infected with two laboratory strains of M. tuberculosis, H37Ra and H37Rv (MOI 1:1) or were cultured with LPS (10 μg/ml) from E. coli. Control alveolar macrophages were incubated in complete media alone. Levels of guinea pig IL-8 protein were measured in the supernatants recovered from control, LPS-stimulated (A), H37Ra-infected (B) , and H37Rv-infected (C) alveolar macrophages isolated from nonvaccinated (open bars) and BCG-vaccinated (solid bars) guinea pigs by using the human IL-8 ELISA. Data are reported as means ± standard errors of the means. There were four animals per treatment group. Differences between groups were compared by Student's one-tailed t test, assuming equal variances. P values of <0.05 (*) were considered significant.