TABLE 1.
Cryptococcus mannoproteins with Ser/Thr-rich and GPI-anchor domains similar to the C-terminal 88 aa of mp88
| Protein | Size (aa) | cDNA contigb
|
Ser/Thr-rich regiond (%Ser, Thr) | Other conserved domains, patterns, and commentse | |
|---|---|---|---|---|---|
| JEC21 | H99 | ||||
| MP88 | 388 | 1623 | ESTs | 286-361 (46) | None |
| MP-A | 401 | 1283 | ESTs | 298-377 (55) | None, 58% identity with MP88 (aa 23-284) |
| MP-B | 444 | None | None | 324-410 (61) | None, 34% identity with MP88 (aa 45-287) |
| MP-C | 421 | None | EST | 276-398 (35) | None, 31% identity with MP88 (aa 50-274) |
| MP98 | 458a | 1494c | ESTs | 380-432 (64) | Chitin deacetylase catalytic |
| MP-D | 309 | None | 428 | 216-281 (53) | Phosphatidylethanolamine-binding protein; Antifreeze-Type I |
| MP-E | 221 | 1628 | 434 | 123-204 (61) | Antifreeze-Type I |
| MP-F | 213 | 1151 | None | 52-176 (40) | Antifreeze-Type I |
| MP-G | 226 | 223c | 373 | 141-200 (63) | Antifreeze-Type I |
| MP-H | 251 | 1126 | 426 | 93-227 (47) | Antifreeze-Type I, 57% identity with MP-G (aa 19-107) |
| MP-I | 197 | 1579 | 438 | 96-171 (59) | Antifreeze-Type I, 46% identity with MP-G (aa 1-103) |
| MP-J | 197 | 1570 | ESTs | 106-170 (72) | Antifreeze-Type I, 57% identity with MP-G (aa 9-103) |
Based on GenBank accession no. AF361369.
Contig numbers are from data release 11/20/01 for strain JEC21 and data release 12/3/99 for strain H99 by the Oklahoma cDNA Sequencing Project (http://www.genome.ou.edu/cneo.html). Where contigs have not been assembled, cDNA may have been partially sequenced and listed as “ESTs” or else not sequenced by the data release date (indicated by “none”).
Sequence is not full length.
Ser/Thr-rich regions were identified as Ser-rich by InterProScan (http://www.ebi.ac.uk/interpro/scan.html) and reevaluated to include flanking Thrs. Each region is indicated by amino acid positions; the percent amino acids that are Ser or Thr in a defined region is given in parentheses.
Domains and patterns were identified by InterProScan. The percent identities were the results of BLAST 2 Sequence comparisons (National Center for Biotechnology Information).