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. 2026 Jan 12;13:1698030. doi: 10.3389/fcell.2025.1698030

FIGURE 2.

Group of four images depicting HER2 expression analysis in different cell types. (A) Flow cytometry histograms showing HER2 expression across eight cell lines compared to isotype control. (B) Overlay of histograms from different cell lines indicating HER2 levels. (C) Bar graphs comparing relative expression of HER2 in cell lines, showing increased expression in SKBR3 and SKOV3. (D) Fluorescent microscopy image of SKOV3-mCherry cells, displaying red fluorescence, indicating protein expression.

HER2 Expression Profiling in Tumor Cell Lines and Generation of Fluorescently Labeled SKOV3-mCherry Cells. (A,B) Flow cytometry analysis of surface HER2 protein expression in various tumor cell lines. Isotype controls were used to define gating and exclude nonspecific signals. (C) qRT-PCR analysis of HER2 transcript levels in tumor cell lines. β-actin served as the housekeeping gene for normalization. Each group included three independent biological replicates (N = 3). Data are presented as mean ± SEM. (D) Representative fluorescence microscopy image of SKOV3 cells stably expressing mCherry. Scale bar = 100 µm. The schematic figures were created by using BioRender.com. BioRender export ID: CH296KA1AE.