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Effects of E30480 and VT1 on NF-κB DNA binding, IκBα degradation, and IKKα activity in Vero cells. Vero cells were stimulated with E30480 supernatants and VT1 for the times indicated and then assayed for DNA binding activity (A), IκBα content (B and D), and IKKα activity (C) as outlined in Materials and Methods. Each autoradiograph represents at least three experiments. Error bars in graphs indicate standard errors of the mean.
