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. 2005 Nov 15;2(12):e381. doi: 10.1371/journal.pmed.0020381

Figure 3. DC-SIGN Is Induced on Resident Alveolar Mφs upon M. tuberculosis Infection.

Figure 3

(A) Flow cytometry analysis of CD11b and DC-SIGN expression by PBMCs from a healthy donor (upper panels) and a patient with TB (lower panels).

(B) Adherent DC-SIGN− alveolar Mφs from a non-tuberculous patient were infected with a GFP-expressing strain of M. tuberculosis at a MOI of one bacterium per cell, or treated with IL-4, TNF-α, or LPS, or left untreated (ø control). After 48 h at 37 °C, cells were recovered and DC-SIGN expression was analyzed by flow cytometry. In M. tuberculosis panel, the grey area corresponds to GFP+ (infected) cells, and the plain line corresponds to GFP− (uninfected) cells.

(C) Cells infected with M. tuberculosis at a MOI of 1 for 1, 2, 4, or 24 h were analysed by RT-PCR for DC-SIGN and GAPDH mRNAs.