Table 2-.
Commercially Available Antibodies for Various p-tau Species and Their Established Cell Types, References, and Effects
| Antibody Name (Clone) | Established Cell Types | References | Effect of Phosphorylation |
|---|---|---|---|
| TNT-1 | Neurons, Brain Lysate | Fujita Y, doi: 10.1016/j.xpro.2020.100111. PMID: 33377007; PMCID: PMC7756915 Davtyan H,doi: 10.1016/j.vaccine.2017.03.020. Epub 2017 Mar 18. PMID: 28320590 |
Detection total tau independent of their PTMs |
| Tau-5 | Neurons | Sadick JS, doi: 10.1038/srep33999. PMID: 27666089; PMCID: PMC5036045 Qi ZP, doi: 10.4103/1673-5374.177744. PMID: 27073389 |
Oligomeric tau |
| p-tauSer214 | HEK293, SHSY5, Pulmonary Endothelial Cells | Wu R. doi: 10.3389/fnmol.2021.631833. PMID: 34054426; PMCID: PMC8155256 Farrell K, doi: 10.1007/s00401-021-02379-z. Epub 2021 Nov 1. PMID: 34719765 |
Detachment of tau from microtubules leading to endothelial cell barrier disruption |
| p-tauSer262 | HEK293,N2a (Neuronal) Cell line | Liu P. doi: 10.1155/2018/2025914. PMID: 30057671; PMCID: PMC6051032 Sun ZD. doi: 10.4103/1673-5374.336872. PMID: 35259851 |
Reduced affinity for microtubules and detachment from microtubules |
| p-tauSer199 | Neurons | Johnson GV, doi: 10.1242/jcs.01558. PMID: 15537830 Jin N. doi: 10.1074/jbc.M115.645507. Epub 2015 Apr 27. PMID: 25918155 |
Convert tau to an inhibitory molecule that sequesters normal microtubule-associated proteins from microtubules |
| p-tauSer396 | Neurons | Di Primio C, doi: 10.1093/pnasnexus/pgad282. PMID: 37731949 Yao SY.doi: 10.1111/bph.16048. Epub 2023 Feb 28. PMID: 36727262 |
Significantly increased propensity to aggregate |
| p-tauThr231 | HEK293, N2a | Levert S, doi: 10.1007/s12035-022-03121-w. Epub 2022 Nov 18. PMID: 36399251 Jadiya P. doi: 10.1016/j.isci.2023.106296. PMID: 36936788 |
• PHF-Tau and tangles • trans-to-cis isomerization, leading to a conformational change that reduces the affinity of the protein for microtubules |