Figure 2: Proximal tubular injury is diminished with reduced capillary regression.

(A) Representative H&E-stained kidney sections from the indicated genotypes 10 days after UUO. Asterisks (*) denote dilated tubules. Scale bar = 50 μm. (B) Representative immunofluorescence images of UUO kidneys stained for KIM-1 (green) and and counterstained with the nuclear dye Hoechst (white). Top row: low magnification (2X) whole-kidney images; scale bar = 1000 μm. Bottom row: high magnification images (20X); scale bar = 200 μm. (C) Quantification of KIM-1⁺ area normalized to total tubular area across genotypes. Each dot represents an individual animal; N=3–5. 3–4 images were quantified per animal. Data are presented as mean ± SEM. *p-values indicate significant differences between DKO UUO group versus other UUO groups. Data were analyzed using one-way ANOVA and post hoc comparisons.