Figure 3: Loss of TNFα and endothelial IL-1R1 limits renal fibrosis.
(A) Representative polarized light image of a contralateral kidney stained with Picrosirius Red. Scale bar = 50 μm. (B) Representative polarized light images of Picrosirius Red-stained UUO kidneys across genotypes. Scale bar = 50 μm. (C) Quantification of percent collagen area in UUO kidney across all genotypes. 8 ROI quantified per mouse. (D) Quantification of collagen fiber maturity in UUO kidneys from indicated genotypes. 6 ROI quantified per mouse. Mature (red) and immature (green) fibers are expressed as relative content of total fibers. Data are presented as mean ± SEM. *p-values indicate significant differences between DKO UUO group versus UUO groups across other genotypes. Data were analyzed using one-way ANOVA and post hoc comparisons
