Figure 4: The myofibroblast marker αSMA is enhanced in mice lacking both TNFα and endothelial IL-1R1 signaling after UUO.

(A) Representative immunofluorescence images of kidney sections stained for αSMA (red) and Hoechst (blue) from contralateral and UUO kidneys of indicated genotypes 10 days after UUO. Scale bar = 200 μm. (B) Quantification of αSMA-positive area (% of total area). Each dot represents an individual animal; N=3–5. 3–4 images were quantified per animal. Data are presented as mean ± SEM. *p-values indicate significant differences between DKO UUO group versus UUO groups across other genotypes, # denotes p<0.05 between contralateral and UUO kidneys within each genotype. Data were analyzed using two-way ANOVA and post hoc comparisons.