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PCR analysis of B. thuringiensis wild type and isogenic mutants. Chromosomal DNA from wild-type strain BT407 and mutant strains BTplcA::lacZ (ΔplcA), and BTplcB::lacZ (ΔplcB), were used to amplify plcA, plcB, or lacZ, as indicated in the figure. PCR products were of the predicted sizes (indicated by molecular weights [in thousands] on the left).
