TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant properties | Reference or source |
|---|---|---|
| Strains | ||
| BT407 | Wild-type B. thuringiensis, Cry− | 27 |
| BTplcA::lacZ | BT407 with lacZ insertion into plcA | This work |
| BTplcB::lacZ | BT407 with lacZ insertion into plcB | This work |
| E. coli TG1 | [Δ(lac-proAB) supE thi hdsD5 (F′ traD36 proA+proB+lacIqZΔM15)] | 16 |
| E. coli SCS110 | [rpsL thr leu endA thi-1 lacY galK galT ara tonA tsx dam dcm supE44 Δ(lac-proAB) (F′ traD36 proAB lacIqZΔM15)] | 16 |
| Plasmids | ||
| pHT304-18Z | Source of promotorless lacZ for insertional mutagenesis of BT407 | 1 |
| pRN1501 | Temperature-sensitive vector containing the ori(Ts) and erm of pE194ts and the oriEc, amp, and multicloning region of pBR322 | 28, 44 |