TABLE 2.
PCR primers used in this study
| Primer | Sequencea | Restriction site |
|---|---|---|
| PlcA1 | 5′-GTAGATACAACTACAGCTGAAC | |
| PlcA2 | 5′-GCTCTAGACACTTTTTCTGTTTTTACATC | XbaI |
| PlcA3 | 5′-GTATGGGGAATGACGCAAG | |
| PlcA4 | 5′-GCGGATCCTTGAATTCCCAGCCTACTC | BamHI |
| PlcB1 | 5′-CCCAAGCTTGTTTAGATAAGCCTTAATAATAGT | HindIII |
| PlcB2 | 5′-CGGAATTCCATTGTCTGGATCATAGAAATG | EcoRI |
| PlcB3 | 5′-CGGAATTCCATTTGCAAAGCAGGCAAAAG | EcoRI |
| PlcB4 | 5′-CGGGATCCGTACGTATCAAACCAAAGC | BamHI |
| LacZ-F | 5′-GGTTAAATTGCCAACGCTTAATTAAC | |
| LacZ-R | 5′-GTTATCTGGAAGATCAGGATATGTGG |
a
The restriction site is underlined.