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. 2005 Oct 28;102(45):16239–16244. doi: 10.1073/pnas.0503137102

Fig. 4.

Fig. 4.

Immunoprecipitation analyses of overexpressed EN and its interaction with TβRII proteins in 293T cells. (a) Schematic representation of full-length and truncated TβRII proteins is shown in Upper.(b and c) Immunoprecipitation and immunoblot analyses of Flag-tagged TβRII proteins (b) or Myc-tagged TβRII protein (c) interacting with V5-tagged EN. (d) Comparison of the cytoplasmic segment (residues 244–295) of the TβRII with representative sequences of ActRIIA, ActRIIB, and BMPRII. (e) Identification of residues critical for EN/TβRII. TβRII (TβRII Δ267–271) mutant, which deleted residues 269–271, was generated and examined its ability to interact with EN in 293T cells. (f) EN does not suppress TβRIIΔ267–271-mediated transcriptional activity in 293T cells.