Fig. 3.
Western blot analysis. Total (Total), cytosolic (Cyto), chloroplastic (Chloro), or mitochondrial (Mito) proteins extracts were prepared from A. thaliana suspension cells. Immunodetection was performed with bean chloroplastic leucyl-tRNA synthetase (@LeuRS) (19), A. thaliana alanyl-tRNA synthetase (@AlaRS-1) (7), maize mitochondrial pyruvate dehydrogenase (@PDH E1-α) (GT Monoclonal Antibodies), Chlamydomonas chloroplastic light harvesting complex II (@LHC II) and α-tubuline (@α-tubuline) (Amersham Pharmacia Biotech).