Table 1. Fifty-three predicted genes with similarities to aminoacyl-tRNA synthetases were identified in A. thaliana.
| Amino acid | AGI no. | EST, or whole-genome array* | Full-length mRNA | Potential targeting sequence | Closest similarity to aaRSs from |
|---|---|---|---|---|---|
| Ala | At1g50200 | + | − | + | Cytosol |
| At5g22800 | + | − | + | Cyanobacteria | |
| Cys | At5g38830† | + | − | Eubacteria‡ | |
| At3g56300† | + | − | Eubacteria‡ | ||
| At2g31170† | + | + | + | Eubacteria‡ | |
| Asp | At4g33760 | + | + | + | Cyanobacteria |
| At4g31180† | + | + | Cytosol | ||
| At4g26870† | + | + | Cytosol | ||
| Glu | At5g26710 | + | + | Cytosol | |
| At5g64050 | + | + | + | Cyanobacteria | |
| Phe | At4g39280 | + | + | Cytosol | |
| At1g72550 | + | + | Cytosol | ||
| At3g58140 | + | + | + | Mitochondria | |
| Gly | At3g48110 | + | + | + | Eubacteria |
| At1g29880 | + | + | + | Cytosol | |
| At3g44740 | +*§ | − | |||
| At1g29870 | − | − | |||
| His | At3g02760 | + | − | Cytosol | |
| At3g46100 | + | + | + | ArChaea | |
| Ile | At5g49030 | + | + | + | Cyanobacteria |
| At4g10320 | + | − | Cytosol | ||
| Lys | At3g11710 | + | + | Cytosol | |
| At3g13490 | + | + | + | Cyanobacteria | |
| Leu | At4g04350 | + | + | + | Eubacteria |
| At1g09620 | + | + | Cytosol | ||
| Met | At4g13780 | + | + | Cytosol | |
| At3g55400 | + | + | + | Cyanobacteria | |
| At2g40660 | +§ | + | |||
| Asn | At5g56680† | + | + | Cyanobacteria | |
| At4g17300† | + | + | + | Cyanobacteria | |
| At1g70980† | + | − | Cyanobacteria | ||
| At3g07420 | +§ | + | |||
| At1g68420 | +*§ | − | |||
| At5g38750 | − | − | |||
| Pro | At5g52520 | + | − | + | Cytosol |
| At3g62120 | + | + | Cytosol | ||
| Gln | At1g25350 | + | + | Cytosol | |
| At5g19720 | +*§ | − | |||
| Arg | At4g26300 | + | − | + | Cytosol |
| At1g66530 | + | + | Cytosol | ||
| Ser | At5g27470 | + | + | Cytosol | |
| At1g11870 | + | + | + | Mitochondria | |
| Thr | At5g26830† | + | + | + | Cytosol |
| At2g04842 | +* | − | + | Cyanobacteria | |
| At1g17960† | + | + | Cytosol | ||
| Val | At5g16715 | + | − | + | Eubacteria |
| At1g14610 | + | + | + | Cytosol | |
| At1g27160 | +*§ | − | |||
| Trp | At3g04600 | + | + | Cytosol | |
| At2g25840 | + | + | + | Cyanobacteria | |
| Tyr | At3g02660 | + | − | + | Eubacteria |
| At2g33840† | + | + | Archaea | ||
| At1g28350† | + | − | Archaea |
Identification codes are from the Arabidopsis Genome Initiative annotation at The Arabidopsis Information Resource (http://arabidopsis.org).
Whole-genome array. Indications about the expression of these genes [full-length mRNA, EST, whole genome array] and the aaRS domains were obtained from http://mips.gsf.de, http://arabidopsis.org, www.tigr.org, www.ncbi.nlm.nih.gov, and www.rarf.riken.go.jp. Similarities to proteins from other organisms were estimated from distance matrices by following clustalw alignments to all other annotated aminoacyl-tRNA synthetases in SwissProt (29).
Close paralogues resulting from recent duplications.
Weak similarities (see also ref. 8).
In italics are the two genes for which no expression could be found and the six others that we predict to be pseudogenes, or at least to encode proteins with no aaRS activity because they lack essential aaRS domains.