Table 2. Twenty-three aaRS sequences have a potential mitochondrial and/or chloroplastic targeting sequence.

Amino acid	Mitochondrial tRNA		predotar		targetp		mitoprot	Experimental localization
		AGI no.	Mit	Chloro	Mit	Chloro	Mit	Mit	Chloro	Ref. and remarks
Cys	Native	At2g31170	0.21	0.70	0.40	0.80	0.97	+	+	8
Met	Native	At3g55400	0.35	0.93	0.19	0.83	0.91	+	+	6
Asn	cp-like	At4g17300	0.29	0.97	0.35	0.83	1.00	+	+	8
His	cp-like	At3g46100	0.50	0.01	0.59	0.22	0.99	+	+	4
Glu	Native	At5g64050	0.37	0.07	0.77	0.29	1.00	+	+	—
Lys	Native	At3g13490	0.26	0.56	0.51	0.49	0.89	+	+	—
Pro	Native	At5g52520	0.36	0.19	0.63	0.72	0.94	+	+	—
Tyr	Native	At3g02660	0.13	0.84	0.19	0.86	0.98	+	+	—
Ser	Native + cp-like	At1g11870	0.48	0.00	0.52	0.09	0.99	+	+	—
Asp	cp-like	At4g33760	0.50	0.01	0.32	0.36	0.99	+	+	—
Ala	Imported	At1g50200	0.69	0.02	0.70	0.74	0.99	+	+	AlaRS-1:also cyto (7)
		At5g22800	0.04	0.00	0.19	0.63	0.87	+	+	AlaRS-2
Phe	Imported	At3g58140	0.39	0.48	0.16	0.76	0.94	+	+	—
Leu	Imported	At4g04350	0.01	0.03	0.25	0.92	0.83	−	+	LeuRS-2
Arg	Imported	At4g26300	0.04	0.61	0.10	0.91	0.74	−	+	—
Thr	Imported	At5g26830	0.91	0.00	0.72	0.31	1,00	+	−	ThrRS-1:also cyto (9)
		At2g04842	0.18	0.96	0.14	0.89	1.00	+	+	ThrRS-2
Val	Imported	At1g14610	0.88	0.04	0.84	0.24	0.99	+	−	ValRS-1:also cyto (9)
		At5g16715	0.79	0.60	0.11	0.94	1.00	?	+	ValRS-2
Trp	Imported	At2g25840	0.18	0.39	0.27	0.85	0.96	+	+	—
Gly	Imported + native	At1g29880	0.41	0.01	0.91	0.04	0.92	+	−	GlyRS-1:also cyto (5)
		At3g48110	0.09	0.15	0.22	0.82	0.99	+	+	GlyRS-2 (5)
Ile	Imported + native	At5g49030	0.07	0.52	0.13	0.77	0.55	?	+

The origin of their cognate mitochondrial tRNAs is indicated. The targeting predictions obtained with different programs are indicated, as well as the experimental results. Mit, mitochondrial; Chloro, chloroplastic.