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. 2005 Oct 26;102(45):16221–16226. doi: 10.1073/pnas.0505089102

Table 2. Prediction of residue burial and active-site proximity from Ala and Asp scanning mutagenesis.

Ala*
Asp*
Class 0% 0.1% 0% 0.1% Burial prediction No. of positions Accuracy, %
1 A A A A Exposed, not active site 40 90
2 A A I A Partially exposed, not active site 5 80
3 A A I I Buried, not active site or exposed, active site§ 7 86
4 I A I A Partially exposed 1 100
5 I A I I Buried, not active site 9 77
6 I I I I Active site proximal 4 100
*

0% and 0.1% refer to phenotypes of Ala or Asp mutants at 0% and 0.1% arabinose, respectively. A and I refer to active and inactive phenotype, respectively

Represents exposed residues with an ACC > 5%

Represents partially exposed residues with an ACC of 5–15%. Exposed residues can be subdivided into partially (ACC 5–15%) and completely (ACC > 15%) exposed categories

§

For buried positions, the corresponding Asp mutant will show substantial reductions in protein expression and/or solubility. If the residue is an exposed hydrophobic that is part of the active site, expression and solubility will not be affected