Table 3. Solubility and activity of aspartate mutants of CcdB in CSH501 as a function of ACC.

ACC, %	Total no. of residues	No. soluble	No. insoluble	No. active (0.1% arabinose)
0–5	16	1	15	0
5–15	15	12	3	13
15–40	38	38	0	34

Soluble and insoluble indicate whether protein is present in supernatant or pellet, respectively, after cell lysis.