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. 2005 Oct 31;102(45):16385–16390. doi: 10.1073/pnas.0506081102

Fig. 1.

Fig. 1.

Scheme of triparental matings. Bacteria are represented by ovals containing the respective plasmids. Antibiotic resistance of strains and mobilizable plasmids is indicated. Plasmid pSU1371, containing R388-oriT, is mobilized from strain 1 by nonmobilizable helper plasmids pET29:trwABC (providing R388 trwA, trwB, and trwC genes) and pKM101Δmob (providing the T4SS). TrwC is represented by a diamond, leading or not the transferred DNA (wavy line) into a recipient cell. TrwC in strain 2 is able or not to complement the R388 mutants (strain 2, left and right, respectively), leading to transconjugants or lack thereof in strain 3 (strain 3 left and right, respectively). Nx, nalidixic acid; Sm, streptomycin; Rf, rifampicin.