Table 1. Plasmids used in this work.
| Plasmid | Description | Phenotype* | Source |
|---|---|---|---|
| pBBR::oriT | R388 oriT in pBBR6 | Gm | This work |
| pClomob::oriT | pSU4814::R388 oriT | Cm | This work |
| pET29c | Expression vector | Km | Novagen |
| pET29::trwABC | PABC-trwABC† in pET29c | Km | This work |
| pET29::trwAC | PABC-trwA-trwC† in pET29c | Km | This work |
| pET3a | Expression vector | Ap | Novagen |
| pET3::trwAC | PABC-trwA-trwC† in pET3a | Ap | This work |
| pET3::trwACmut | As pET3::trwAC, TrwC Y18FY26F | Ap | This work |
| pKK223-3 | Expression vector | Ap | Pharmacia |
| pKK::oriT | pKK223–3:R388 oriT | Ap | This work |
| pKK::oriT-Km | pKK::oriT plus nptII gene | Ap Km | This work |
| pKM101Δmob | pKM101 SmaI deletion derivative‡ | Ap | This work |
| pR6K::oriTW | oriV(R6K)::R388 oriT + nptII gene | Cm | This work |
| pR6K::oriTWoriTP | pR6K::oriTW plus RP4 oriT | Cm | This work |
| pRec2oriT-Ap | oriT recombination substrate | Ap Km | This work |
| pRec2oriT-Cm | oriT recombination substrate | Cm Km | This work |
| pSU711ΔoriT::aac3 | R388 derivative without oriT | GmKm | 22 |
| pSU1371 | R388 oriT in pSU19 | Cm | 23 |
| pSU1443 | R388 trwB | Tp Km | 21 |
| pSU1445 | R388 trwC | Tp Km | 21 |
| pSU1458 | R388 trwC | Tp | 21 |
| pSU1547 | pET22::trwA | Ap | 24 |
| pSU4028 | R388 trwA | Cm | 25 |
| pSU4058 | R388 T4SS in vector pHG329 | Ap | 25 |
| pSU4814 | pSU19::CloDF13mob | Cm | 26 |
*
Antibiotic resistance: Ap, ampicillin; Cm, chloramphenicol; Gm, gentamicin; Km, kanamycin; Tp, trimethoprim
†
PABC, R388 promoter for the trwA-trwB-trwC operon
‡
This plasmid retains only the T4SS component of its transfer system